At the 2023 Pan American Games opening ceremony on October 20, athletes proudly carried their national flags, leading their delegations into Santiago’s National Stadium during the Parade of Nations. In a move promoting gender equality, echoing a precedent set at the 2020 Summer Olympics, each team had the choice to have both a male and female flag bearer for the first time.
Athletes marched into the stadium following a traditional sequence. Argentina, having hosted the inaugural Pan American Games, made its entrance first. As the hosting country, Chile’s team entered last. All other nations proceeded in line with the Spanish alphabetical order, in accordance with the Pan American Sports Organization’s guidelines.
2023 Pan American Games Parade of Nations Flag Bearers
| Order | Nation | Spanish | Flag bearer | Sport |
| 1 | Argentina | Argentina | Marcos Moneta | Rugby sevens |
| Sabrina Ameghino | Canoeing | |||
| 2 | Antigua and Barbuda | Antigua y Barbuda | ||
| 3 | Aruba | Aruba | ||
| 4 | Bahamas | Bahamas | ||
| 5 | Barbados | Barbados | ||
| 6 | Belize | Belice | ||
| 7 | Bermuda | Bermuda | ||
| 8 | Bolivia | Bolivia | Conrrado Moscoso | Racquetball |
| Noelia Zeballos | Tennis | |||
| 9 | Brazil | Brasil | ||
| 10 | Canada | Canadá | ||
| 11 | Colombia | Colombia | Miguel Ángel Trejos | Taekwondo |
| Jenny Arias | Boxing | |||
| 12 | Costa Rica | Costa Rica | Andrés Acuña | Racquetball |
| Nishy Lee Lindo | Taekwondo | |||
| 13 | Cuba | Cuba | Julio César La Cruz | Boxing |
| Idalys Ortiz | Judo | |||
| 14 | Dominica | Dominica | ||
| 15 | Ecuador | Ecuador | Daniel Pintado | Athletics |
| Angie Palacios | Weightlifting | |||
| 16 | El Salvador | El Salvador | Amado de Jesús Alvarado | Surfing |
| Sindy Portillo | Surfing | |||
| 17 | United States | Estados Unidos de América | ||
| 18 | Grenada | Granada | ||
| 19 | Guatemala | Guatemala | ||
| 20 | Guyana | Guyana | ||
| 21 | Haiti | Haití | ||
| 22 | Honduras | Honduras | Julio Horrego | Swimming |
| Natalie Espinal | Tennis | |||
| 23 | Cayman Islands | Islas Caimán | ||
| 24 | Virgin Islands | Islas Vírgenes | ||
| 25 | British Virgin Islands | Islas Vírgenes Británicas | ||
| 26 | Jamaica | Jamaica | ||
| 27 | Mexico | México | Carlos Sansores | Taekwondo |
| Karina Esquer | Basketball | |||
| 28 | Nicaragua | Nicaragua | ||
| 29 | Panama | Panamá | Davis Romero | Baseball |
| Hillary Heron | Gymnastics (artistic) | |||
| 30 | Paraguay | Paraguay | Derlis Ayala | Athletics |
| Agua Marina Espínola | Cycling | |||
| 31 | Peru | Perú | Nicolás Pacheco | Shooting |
| Gladys Tejeda | Athletics | |||
| 32 | Puerto Rico | Puerto Rico | Jean Pizarro | Archery |
| María Pérez | Judo | |||
| 33 | Dominican Republic | República Dominicana | Robert Pigozzi | Water skiing |
| Yvonne Losos de Muñiz | Equestrian | |||
| 34 | Saint Kitts and Nevis | San Cristóbal y Nieves | ||
| 35 | Saint Lucia | Santa Lucía | Luc Chevrier | Sailing |
| 36 | Saint Vincent and the Grenadines | San Vicente y las Granadinas | ||
| 37 | Suriname | Surinam | ||
| 38 | Trinidad and Tobago | Trinidad y Tobago | ||
| 39 | Uruguay | Uruguay | Rodrigo Chagas | Football |
| Camila Piñeiro | Boxing | |||
| 40 | Venezuela | Venezuela | Keydomar Vallenilla | Weightlifting |
| Joselyn Brea | Athletics | |||
| 41 | Chile | Chile | Esteban Grimalt | Beach volleyball |
| Kristel Köbrich | Swimming |